Preparation and characterization of antigen and antibody absorbents covalently coupled to an inorganic carrier

摘要

Immunoadsorbents were prepared by the use of an inorganic carrier. The proteins were covalently coupled to the carrier through a silane coupling agent. An immunoadsorbent prepared with human γ-globulin was found to complex with specific antibodies. These antibodies could be recovered in high yield and purity from the adsorbent. Similarly, insolubilized antibodies to l-asparaginase were used to isolate and purify the enzyme from crude extracts. These insolubilized antibodies are extremely stable with time and may be reused many times.